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Bozzini was fascinated with creating an instrument that would allow a physician to look into the inner cavities of the human body. He first presented his idea, the Lichtleiter or "Light Conductor" to the public in 1804 and officially on February 7, 1805. In July of 1806 the instrument was demonstrated at a scientific session in Frankfurt, and it was noted that the application of his instrument for the inspection of the pharynx and the nasal cavities was indeed remarkable. Bozzini presented his first publication in 1806 in Hufeland's Journal of Practical Medicine, Volume 24, under the title "Light Conductor, An Invention for the Viewing of Internal Parts and Diseases with Illustration." There were many fans and critics of the instrument, and decades later Bozzini's invention was honored—in 1876 it was mentioned as the first laryngoscope.

After saving 42 of his patients suffering from typhoid fever, Bozzini succumbed to it himself on April 4, 1809.

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